Journal: JCI Insight

Article Title: Lymph node CXCR5 + NK cells associate with control of chronic SHIV infection

doi: 10.1172/jci.insight.155601

Figure Lengend Snippet: ( A ) Gating strategy for CXCR5-expressing NK cells is shown. Frequency of NK cells ( B ) and CXCR5-expressing NK cells ( C ) in blood ( n = 17) and lymph nodes (LNs) ( n = 17) of uninfected macaques. Proportions of total NK cells ( D ), CXCR5 + ( E ), and CXCR5 – NK cells ( F ) at preinfection day 0 ( n = 11) and week 14 of SHIV infection ( n = 21). ( G–I ) IHC images from 3 representative SHIV-infected (14 weeks after infection) macaques showing CD20 (blue), CD3 (green), NKG2A (red), and CXCR5 (gray) staining of LN sections ( G ). Mean number of NK cells per mm square area of B cell follicle (BCF) and T cell zone (TCZ) compartments of LN sections from 3 macaques ( H ). MFI ± SEM of CXCR5 on NKG2A + cells ( n = 46) in BCF and TCZ ( I ). P value was calculated using Mann-Whitney test. Scale bar: 50 μm. Wilcoxon’s matched-pairs signed rank test was used to compare the frequencies of CXCR5 + NK cells between blood and LN. Arrows indicate NK cells. **** P < 0.0001.

Article Snippet: The primary antibodies contained rat anti-human CD3 (Bio-Rad, MCA1477), rabbit anti-NKG2A (Abcam, ab93169), goat anti-hCD20 (LSBio, LS-B11144), and mouse anti-CXCR5 (NIH), or mouse α granzyme B (clone GB11, Invitrogen, MA1-80734).

Techniques: Expressing, Infection, Staining, MANN-WHITNEY

Journal: JCI Insight

Article Title: Lymph node CXCR5 + NK cells associate with control of chronic SHIV infection

doi: 10.1172/jci.insight.155601

Figure Lengend Snippet: ( A ) Purified NK cells from lymph nodes (LNs) at week 14 after infection were RNA sequenced, and volcano plots were analyzed for differentially expressed transcripts ( n = 4). ( B ) Normalized enrichment scores for upregulated gene sets in CXCR5 + NK cells are depicted. Dashed line indicates normalized enrichment score cutoff of greater than 135 with FDR of less than 0.2. ( C ) GSEA plots comparing CXCR5 + and CXCR5 – NK cells. ( D ) Global gene expression analysis showing gene expression profile for CXCR5 + and CXCR5 – NK cells. The color intensity for heatmaps represents Z score of differential expression by CXCR5 + versus CXCR5 – NK cells, calculated as described in Methods. ( E ) IHC images showing CD20 (blue), CD3 (green), NKG2A (red), and granzyme B (gray) staining of LN sections of SHIV-infected macaques. Scale bar: 50 μm. Arrow indicates NK cells.

Article Snippet: The primary antibodies contained rat anti-human CD3 (Bio-Rad, MCA1477), rabbit anti-NKG2A (Abcam, ab93169), goat anti-hCD20 (LSBio, LS-B11144), and mouse anti-CXCR5 (NIH), or mouse α granzyme B (clone GB11, Invitrogen, MA1-80734).

Techniques: Purification, Infection, Gene Expression, Quantitative Proteomics, Staining

Journal: Lipids in Health and Disease

Article Title: C-reactive protein/oxidised low-density lipoprotein/β2-glycoprotein I complex promotes atherosclerosis in diabetic BALB/c mice via p38mitogen-activated protein kinase signal pathway

doi: 10.1186/1476-511X-12-42

Figure Lengend Snippet: Effect of CRP / oxLDL / β2 - GPI on infiltration of inflammatory cells in the aortas. Aortas were removed, paraffin-embedded, deparaffinated, underwent antigen retrieval, blocked, and then incubated with primary antibodies (Mac 1:200 or α- SMA 1:100 or CD3 1:100) at 4°C overnight. Biotinylated secondary antibody (1:200) was added, followed by HRP-labeled streptavidin incubation. Infiltrations of macrophages, SMCs and T cells in the aortas were quantified as the percentage of the luminal surface covered by positive cells to the plaque area using Image Pro-Plus 6.0. Results are shown as means ± SEM. A : Infiltration of SMCs in the aortas, 400× magnification. B : Infiltration of macrophages in the aortas, 400× magnification. C : Infiltration of T cells in aortas, 400× magnification. D : Positive expressions of SMA, MAC and CD3 (n=4), expressed as the percentage to plaque area. * P <0.05, compared with the CRP/oxLDL/β2GPI group, † P < 0.05, compared with the PBS group.

Article Snippet: Mice anti-α-smooth muscle actin (SMA, BM0002) antibody, rabbit anti-CD3 (BA0429) antibody were purchased from Boster, Wuhan, China.

Techniques: Incubation, Labeling